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fluorescein isothiocyanate conjugated avidin fitc avidin  (Vector Laboratories)


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    Structured Review

    Vector Laboratories fluorescein isothiocyanate conjugated avidin fitc avidin
    Fluorescein Isothiocyanate Conjugated Avidin Fitc Avidin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 896 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/avidin+d+fitc+conjugated/Fluorescein+Avidin+D/pmc12736424-166-28-32
    Average 94 stars, based on 896 article reviews
    fluorescein isothiocyanate conjugated avidin fitc avidin - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Cholecystokinin in the central nervous system of the sea lamprey Petromyzon marinus: precursor identification and neuroanatomical relationships with other neuronal signalling systems.
    Article Snippet: .. Then, the sections were incubated at room temperature with Avidin D-FITC conjugated (dilution 1:1,000; Vector; Cat#: A-2001) diluted in TBS containing 0.3% Triton X-100 for 4 h to reveal the presence of neurobiotin. .. Slides were rinsed in TBS and distilled water and mounted with Mowiol (Sigma).

    Article Title: GABA promotes survival and axonal regeneration in identifiable descending neurons after spinal cord injury in larval lampreys
    Article Snippet: Brains of these larvae were dissected out, and the posterior and cerebrotectal commissures of the brain were cut along the dorsal midline, and the alar plates were deflected laterally and pinned flat to a small strip of Sylgard (Dow Corning Co., USA) and fixed with 4% PFA in TBS for 4 h at room temperature. .. After washes in TBS, the brains were incubated at room temperature with Avidin D-FITC conjugated (Vector; Cat#: A-2001; 1:500) diluted in TBS containing 0.3% Triton X-100 for 2 days to reveal the presence of Neurobiotin. ..

    Article Title: Serotonin inhibits axonal regeneration of identifiable descending neurons after a complete spinal cord injury in lampreys
    Article Snippet: The sections were rinsed in TBS and incubated for 1 h at room temperature with a Cy3-conjugated goat anti-rabbit antibody (1:200; Millipore; Burlington, MA). .. Then, the sections were incubated at room temperature with Avidin D-FITC conjugated (Vector; Cat#: A-2001; 1:1000) diluted in TBS containing 0.3% Triton X-100 for 4 h to reveal the presence of Neurobiotin. ..

    Article Title: Taurine promotes axonal regeneration after a complete spinal cord injury in lampreys
    Article Snippet: .. After washes in TBS, the brains/spinal cords were incubated at 4 oC with Avidin D-FITC conjugated (Vector; Cat#: A-2001; 1:500) diluted in TBS containing 0.3% Triton X-100 for 2 days to reveal the presence of Neurobiotin. ..

    Article Title: Organization of the corticotropin-releasing hormone and corticotropin-releasing hormone-binding protein systems in the central nervous system of the sea lamprey Petromyzon marinus.
    Article Snippet: .. After the immunofluorescence protocol, the sections were incubated at room temperature with Avidin D-FITC conjugated (dilution 1:1000; Vector; Cat#: A-2001) diluted in TBS containing 0.3% Triton X-100 for 4 h to reveal the presence of neurobiotin. .. Slideswere rinsed in TBS and distilledwater andmounted withMowiol® (Sigma).

    Article Title: Taurine Promotes Axonal Regeneration after a Complete Spinal Cord Injury in Lampreys.
    Article Snippet: .. After washes in TBS, the brains/spinal cords were incubated at 4 oC with Avidin D-FITC conjugated (Vector; Cat#: A-2001; 1:500) diluted in TBS containing 0.3% Triton X-100 for 2 days to reveal the presence of Neurobiotin. ..

    Article Title: Serotonin inhibits axonal regeneration of identifiable descending neurons after a complete spinal cord injury in lampreys
    Article Snippet: Brains of these larvae were dissected out, the posterior and cerebrotectal commissures of the brain were cut along the dorsal midline, and the alar plates were deflected laterally and pinned flat to a small strip of Sylgard (Dow Corning Co., USA) and fixed with 4% PFA in TBS for 4 h at 4°C. .. After washes in TBS, the brains were incubated at 4°C with Avidin D-FITC conjugated (Vector; Cat#: A-2001; 1:500) diluted in TBS containing 0.3% Triton X-100 for 2 days to reveal the presence of Neurobiotin. ..

    Avidin-Biotin Assay:

    Article Title: Cholecystokinin in the central nervous system of the sea lamprey Petromyzon marinus: precursor identification and neuroanatomical relationships with other neuronal signalling systems.
    Article Snippet: .. Then, the sections were incubated at room temperature with Avidin D-FITC conjugated (dilution 1:1,000; Vector; Cat#: A-2001) diluted in TBS containing 0.3% Triton X-100 for 4 h to reveal the presence of neurobiotin. .. Slides were rinsed in TBS and distilled water and mounted with Mowiol (Sigma).

    Article Title: GABA promotes survival and axonal regeneration in identifiable descending neurons after spinal cord injury in larval lampreys
    Article Snippet: Brains of these larvae were dissected out, and the posterior and cerebrotectal commissures of the brain were cut along the dorsal midline, and the alar plates were deflected laterally and pinned flat to a small strip of Sylgard (Dow Corning Co., USA) and fixed with 4% PFA in TBS for 4 h at room temperature. .. After washes in TBS, the brains were incubated at room temperature with Avidin D-FITC conjugated (Vector; Cat#: A-2001; 1:500) diluted in TBS containing 0.3% Triton X-100 for 2 days to reveal the presence of Neurobiotin. ..

    Article Title: Serotonin inhibits axonal regeneration of identifiable descending neurons after a complete spinal cord injury in lampreys
    Article Snippet: The sections were rinsed in TBS and incubated for 1 h at room temperature with a Cy3-conjugated goat anti-rabbit antibody (1:200; Millipore; Burlington, MA). .. Then, the sections were incubated at room temperature with Avidin D-FITC conjugated (Vector; Cat#: A-2001; 1:1000) diluted in TBS containing 0.3% Triton X-100 for 4 h to reveal the presence of Neurobiotin. ..

    Article Title: Taurine promotes axonal regeneration after a complete spinal cord injury in lampreys
    Article Snippet: .. After washes in TBS, the brains/spinal cords were incubated at 4 oC with Avidin D-FITC conjugated (Vector; Cat#: A-2001; 1:500) diluted in TBS containing 0.3% Triton X-100 for 2 days to reveal the presence of Neurobiotin. ..

    Article Title: Organization of the corticotropin-releasing hormone and corticotropin-releasing hormone-binding protein systems in the central nervous system of the sea lamprey Petromyzon marinus.
    Article Snippet: .. After the immunofluorescence protocol, the sections were incubated at room temperature with Avidin D-FITC conjugated (dilution 1:1000; Vector; Cat#: A-2001) diluted in TBS containing 0.3% Triton X-100 for 4 h to reveal the presence of neurobiotin. .. Slideswere rinsed in TBS and distilledwater andmounted withMowiol® (Sigma).

    Article Title: Taurine Promotes Axonal Regeneration after a Complete Spinal Cord Injury in Lampreys.
    Article Snippet: .. After washes in TBS, the brains/spinal cords were incubated at 4 oC with Avidin D-FITC conjugated (Vector; Cat#: A-2001; 1:500) diluted in TBS containing 0.3% Triton X-100 for 2 days to reveal the presence of Neurobiotin. ..

    Article Title: Serotonin inhibits axonal regeneration of identifiable descending neurons after a complete spinal cord injury in lampreys
    Article Snippet: Brains of these larvae were dissected out, the posterior and cerebrotectal commissures of the brain were cut along the dorsal midline, and the alar plates were deflected laterally and pinned flat to a small strip of Sylgard (Dow Corning Co., USA) and fixed with 4% PFA in TBS for 4 h at 4°C. .. After washes in TBS, the brains were incubated at 4°C with Avidin D-FITC conjugated (Vector; Cat#: A-2001; 1:500) diluted in TBS containing 0.3% Triton X-100 for 2 days to reveal the presence of Neurobiotin. ..

    Article Title: Inhibition of Gamma-Secretase Promotes Axon Regeneration After a Complete Spinal Cord Injury
    Article Snippet: .. The presence of Neurobiotin in whole-mounted brains/spinal cords was detected using Avidin-D-FITC conjugated (Vector; 1:500). .. A spectral confocal microscope (model TCS-SP2; Leica, Wetzlar, Germany) was used to acquire images of the whole-mounted brains/spinal cords.

    Immunofluorescence:

    Article Title: Organization of the corticotropin-releasing hormone and corticotropin-releasing hormone-binding protein systems in the central nervous system of the sea lamprey Petromyzon marinus.
    Article Snippet: .. After the immunofluorescence protocol, the sections were incubated at room temperature with Avidin D-FITC conjugated (dilution 1:1000; Vector; Cat#: A-2001) diluted in TBS containing 0.3% Triton X-100 for 4 h to reveal the presence of neurobiotin. .. Slideswere rinsed in TBS and distilledwater andmounted withMowiol® (Sigma).



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    Image Search Results


     Antisera  used in each group for light microscopy.

    Journal: Frontiers in Neuroanatomy

    Article Title: Morphological investigations of endomorphin-2 and spinoparabrachial projection neurons in the spinal dorsal horn of the rat

    doi: 10.3389/fnana.2022.1072704

    Figure Lengend Snippet: Antisera used in each group for light microscopy.

    Article Snippet: These sections were blocked with 10% normal donkey serum (NDS) for 0.5 h, then subjected to the following incubations with: (1) primary antisera in the dilution medium [5% NDS, 0.3% Triton X-100, 0.05% NaN3 and 0.25% λ-carrageenan in 0.01 M PBS (PBS-NDS, pH 7.4)] overnight at room temperature (RT) and then 72 h at 4°C; (2) secondary antisera in PBS-NDS for 6 h at RT; (3) FITC-conjugated avidin (A-2001, Vector; Burlingame, CA, USA) in PBS containing 0.3% Triton X-100 (PBS-X, pH 7.4) for 3 h at RT.

    Techniques: Microscopy